Composite
PcusC-GFP
Part:BBa_K4385012:Design
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-01)
Copper sensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 826
Design Notes
The copper sensor part is composed of PcusC and GFP. The initiation of the copper-inducible promoter PcusC requires the support of the CusSR two-component membrane associated sensor kinase system, which is present in the strain E. coli XL-1 Blue.
Source
It comes from E. coli genomic DNA.